Description

1×dsDNA HS Assay Kit is a rapid, highly sensitive and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). This kit is highly selective for dsDNA and has good linearity in the range of 0.2 ng-100 ng, the quantitation range is between 10 pg/μL to 100 ng/μL. This kit is easy to operate, providing a ready-to-use working solution that enables simple dsDNA sample quantification on Qubit Fluorometer or Fluorescence Microplate Reader. It is ideal choice for NGS large-scale DNA sample quantification (such as input DNA quantification, DNA library quantification, etc.). This kit is well tolerated to common contaminants such as proteins and salts.

Features

• High sensitive: the concentrations ranging from 10 pg/µ l to 100 ng/µL of dsDNA can be accurately quantified;
• High specificity: this product is highly selective to dsDNA and is not affected by RNA, has good tolerance to some conventional pollutants, such as salt, free nucleotide, protein, solvent, detergent, etc;
• Ready to use: 1x working solution, just add the dsDNA sample to be tested and test it with Qubit fluorometer or Fluorescence Microplate Reader at room temperature

Applications

• dsDNA quantification with Qubit or Microplate Reader
• NGS library quantification

Specifications

Assay	dsDNA Quantitation
Excitation/Emission	480/520
For Use With (Equipment)	Qubit Fluorometer, Microplate Reader
No. of Reactions	100T/500T
Product Line	Qubit Quantitation
Quantitation Range	10 pg/μL to 100 ng/μL
Sample Volume	1 μL to 20 μL
Detection Method	Fluorescent

Components

Components No.	Name	Concentration	12642ES60 (100T)	12642ES76 (500T)
12642-A	1×dsDNA HS Working Solution	1×concentrate in DMSO	50 mL	250 mL
12642-B	dsDNA Standard 1	0 ng/μL in TE buffer	1 mL	5×1 mL
12642-C	dsDNA Standard 2	10 ng/μL in TE buffer	1 mL	5×1 mL

Shipping and Storage

All the components are shipped with ice pack and can be stored at 2-8℃ away from light for one year.

Figures

Figure 1 Dye binding efficiency and fluorescence signal persistence

The fluorescence signal of Cat#12642 reached saturation within 2min and remained stable within 5h.