Hieff NGS™ Ultima DNA Library Prep Kit for MGI®

Details:

Product Description

Hieff NGS™ Ultima DNA Library Prep Kit for MGI® is a new generation library construction kit specially developed and designed for the MGI® high-throughput sequencing platform. As a new upgrade version, the high-quality enzymatic composition and simplified operation process included in this kit can significantly improve the library conversion rate and amplification efficiency. Hieff NGS™ Ultima DNA Library Prep Kit for MGI® has a wide range of sample adaptability, while compatible with FFPE, cfDNA, ChIP DNA and other samples, help to obtain excellent sequencing data. 

1、 Suitable for 500 pg-1μg DNA samples

2、Compatible with cfDNA, FFPE and other low-quality samples

3、 Efficient library conversion rate and amplification efficiency

4、 Excellent library and sequencing data obtainment verified by multi-sample experiments

5、 Strict batch performance and stability and quality control

Product Components

Component Number and Name

13310ES16

13310ES96

13310-A

Endprep Mix

160 μL

960 μL

13310-B

Ligation Enhancer

480 μL

4×720 μL

13310-C

Fast T4 DNA Ligase

80 μL

480 μL

13310-D

2×Ultima HF Amplification Mix

400 μL

4×600 μL

13310-E

Primer Mix for MGI®

80 μL

480 μL

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for one year.

Cautions

1 Operation

1.1 For your safety and health, please wear lab coats and disposable gloves for operation. For research use only!

1.2 Thaw components at room temperature. Once the components are thawed, mix thoroughly by vortexing, spin the tube briefly and place them on ice for later use.

1.3 When preparing the reaction solution of each step, it is recommended to use a pipette to mix well or gently shake. Vigorous shaking may cause a decrease in library output.

1.4 It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

1.5 It is recommended to perform each reaction step in a thermocycler with a heated lid. The thermocycler should be preheated to the set temperature before use.

1.6 Improper operations may very likely cause carry-over contaminations through aerosols, impacting the experiment’s accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.

Citations & References:

[1] Peng R, Lin G, Li L, Li J. Development of a Novel Reference Material for Tumor Mutational Burden Measurement Based on CRISPR/Cas9 Technology. Front Oncol. 2022;12:845636. Published 2022 Apr 28. doi:10.3389/fonc.2022.845636(IF:6.244)

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