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0Overview of UC Modeling
Since 1985, when the first hamster ulcerative colitis model was prepared using dextran sulphate sodium (DSS), a large number of data have been used to prove that the etiology, clinical symptoms, pathological changes and treatment response of DSS colitis model are similar to those of human ulcerative colitis (UC).
The most commonly used types of UC modeling in current research can be roughly divided into: spontaneous model, induction model (chemical drug induction: TNBS trinitrobenzene sulfonic acid, DSS dextran sulfate, etc., immunological method induction, cell transplantation induction) and gene modification model.
DSS Molding Advantages
Dextran Sulfate Sodium Salt (DSS) is a polyanionic derivative, and although the mechanism of its induction of colitis in the model is still not well understood, it is generally believed to be related to macrophage dysfunction, intestinal microbiota dysbiosis, the toxic effect of DSS on the colon epithelium, and the important role of cytokines in the pathogenesis of DSS colitis model.
Compared with various types of UC modeling, DSS has great advantages in making molds:
1. The symptoms are highly similar to those of human UC: it can be used to study the mechanism of colitis and pharmacodynamic studies.
2. High molding rate: free to drink DSS aqueous solution, simple and easy, strong repeatability.
3. A variety of colitis models can be constructed: acute colitis, chronic colitis, and colitis-related cancer (CAC) models can also be constructed in combination with AOM.
4. It is suitable for modeling a variety of animals: mice, rats, zebrafish, pigs, fruit flies, etc.
5. High safety: DSS can be degraded by natural ecosystems and is safe for the environment.
DSS modeling protocol
Figure 1. Mouse colitis modelling protocol
DSS concentration: typically 3-5% for acute phase models and 1-3% for chronic phase models. The concentration can be found according to the experimental needs and the type of animal. This concentration is a mass-to-volume ratio, and after using a drinking water configuration, a 0.22 μM filter membrane is filtered and sterilized.
Data recording: Mouse body weight, fecal viscosity, fecal occult blood and other indicators were recorded every 24 hours.
1. Acute DSS colitis model
Day1: C57BL/6 mice in each group were weighed and labeled. The mice to be modeled were given 3-5% DSS aqueous solution, and the untreated mice were given normal water.
Day3: Mice to be modeled were given fresh 3-5% DSS drinking water.
Day5: Mice to be modeled were given fresh 3-5% DSS drinking water.
Day8: Mice to be modeled were given fresh drinking water without DSS.
2. Chronic DSS colitis model
Day1: C57BL/6 mice in each group were weighed and labeled. The mice to be modeled were given 1-3% DSS aqueous solution, and the mice in the untreated group were given normal water.
Day3: Mice to be modeled were given fresh 1-3% DSS drinking water.
Day5: Mice to be modeled were given fresh 1-3% DSS drinking water.
Day8: Mice to be modeled were given fresh drinking water without DSS.
Day22-Day26: Repeat operations from Day1 to Day5.
Day29: Mice to be modeled were given fresh drinking water without DSS.
Day43-Day47: Repeat operations from Day1 to Day5.
Day50: Mice to be modeled were given fresh drinking water without DSS.
Data of clients
Case 1:
Customer: Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College (Tianjin).
Mouse type: 6~8 weeks male C57BL/6J mice.
Molding method: 1.5% (w/v) DSS for 15 days.
Figure 2: Results of Yeasen DSS (Cat#60316ES) mouse modeling experiments (Yuan Li, et al. Gut Microbes (2019) 789-806. IF=10.245）
Case 2:
Customer: Nanjing Agricultural University.
Mouse type: 8-week-old male C57BL/6 mice weighing 20~22 g.
Molding method: 3% (w/v) DSS for 7 days.
Figure 3: Results of Yeasen DSS (Cat#60316ES) mouse modeling experiments (Xiaona Gao, et al. Journal of Nutritional Biochemistry 83 (2020) 108438. IF=6.048）
Case 3:
Customer: Ocean University of China.
Mouse type: 4-5 weeks old SPF male C57BL/6J mice.
Molding method: 3.5% (w/v) DSS for one week.
Figure 4: Results of Yeasen DSS (Cat#60316ES) mouse modeling experiments (Hao H, et al. Frontiers in Immunology.2021.777147. IF=7.561）
Published articles with our reagents (incomplete statistics)：
[1] Zhong D, Jin K, Wang R, Chen B, Zhang J, Ren C, Chen X, Lu J, Zhou M. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26: e2312275. doi: 10.1002/adma.202312275. Epub ahead of print. PMID: 38277492. (IF: 29.4)
[2] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464. (IF: 16.74)
[3] Tong, L, et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics 2021, 11 (17), 8570-8586. DOI: 10.7150/thno.62046. (IF: 11.56)
[4] Feng X, et al. Yeast Microcapsule Mediated Natural Products Delivery for Treating Ulcerative Colitis through Anti-Inflammatory and Regulation of Macrophage Polarization. ACS Appl Mater Interfaces. 2022 Jul 13;14(27):31085-31098. doi: 10.1021/acsami.2c05642. Epub 2022 Jun 30. PMID: 35770618. (IF: 10.38)
[5] Li X, et al. Discoidin domain receptor 1(DDR1) promote intestinal barrier disruption in Ulcerative Colitis through tight junction proteins degradation and epithelium apoptosis. Pharmacol Res. 2022 Sep;183:106368. doi: 10.1016/j.phrs.2022.106368. Epub 2022 Jul 26. PMID: 35905891. (IF: 10.33)
[6] Jingjing Gan, et al. Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment. Applied Materials Today, Volume 25, 2021, 101231. (IF: 10.04)
[7] Gan J, et al. Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment. Adv Healthc Mater. 2022 Sep;11(17):e2201105. doi: 10.1002/adhm.202201105. Epub 2022 Jun 30. PMID: 35737997. (IF: 9.93)
[8] Dong J, et al. Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models. Cell Rep. 2021 Oct 26;37(4):109886. doi: 10.1016/j.celrep.2021.109886. PMID: 34706245. (IF: 9.42)
[9] Li Y, et al. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes. 2020 Jul 3;11(4):789-806. doi: 10.1080/19490976.2019.1709387. Epub 2020 Jan 13. PMID: 31931652. (IF: 7.74)
[10] Xu M, et al. Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis. Front Immunol. 2022;13:855645. Published 2022 Mar 22. doi:10.3389/fimmu.2022.855645. (IF:7.56)
[11] Wang S, et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021; 14:7107-7130. Published 2021 Dec 20. doi:10.2147/JIR.S344990. (IF:6.92)
[12] Liu C, et al. Angelica oil restores the intestinal barrier function by suppressing S100A8/A9 signalling in mice with ulcerative colitis. Phytomedicine. 2023; 108:154490. doi:10.1016/j.phymed.2022.154490. (IF:6.66)
[13] Wang S, et al. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway. Oxid Med Cell Longev. 2022;2022:4636618. Published 2022 Jan 27. doi:10.1155/2022/4636618. (IF:6.54)
[14] Li Z, et al. A tetrapeptide from maize combined with probiotics exerted strong anti-inflammatory effects and modulated gut microbiota in DSS-induced colitis mice [published online ahead of print, 2022 Nov 14]. Food Funct. 2022;10.1039/d2fo02678c. doi:10.1039/d2fo02678c. (IF:6.32)
[15] Jia X, et al. The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation. Front Pharmacol. 2022;13:1033874. Published 2022 Oct 14. doi:10.3389/fphar.2022.1033874. (IF:5.99)
[16] Luo P, et al. Central administration of human opiorphin alleviates dextran sodium sulfate-induced colitis in mice through activation of the endogenous opioid system. Front Pharmacol. 2022;13:904926. Published 2022 Sep 13. doi:10.3389/fphar.2022.904926. (IF:5.99)
[17] Zhang K, et al. Pharmacokinetic Study of Four Major Bioactive Components of Liandan Xiaoyan Formula in Ulcerative Colitis and Control Rats Using UPLC-MS/MS. Front Pharmacol. 2022;13:936846. Published 2022 Jul 4. doi:10.3389/fphar.2022.936846. (IF:5.99)
[18] Yan G, et al. Immuno-PET Imaging of TNF-α in Colitis Using <sup>89</sup>Zr-DFO-infliximab. Mol Pharm. 2022;19(10):3632-3639. doi:10.1021/acs.molpharmaceut.2c00411. (IF:5.36)
[19] Xiong X, et al. HuanglianGanjiang Tang alleviates DSS-induced colitis in mice by inhibiting necroptosis through vitamin D receptor. J Ethnopharmacol. 2022;298:115655. doi:10.1016/j.jep.2022.115655. (IF:5.19)
[20] Zhu Y, et al. Endometrial Regenerative Cell-Derived Exosomes Attenuate Experimental Colitis through Downregulation of Intestine Ferroptosis. Stem Cells Int. 2022;2022:3014123. Published 2022 Aug 22. doi:10.1155/2022/3014123. (IF:5.13)
Product Order
|
Product name |
Catalog No. |
Size |
Price(＄) |
|
60316ES25 |
25 g |
255 |
|
|
60316ES60 |
100 g |
755 |
|
|
60316ES76 |
500 g |
3455 |
|
|
60316ES80 |
1 kg |
6255 |
