The protocol of Ulcerative Colitis Rat Modeling using Dextran sodium sulfate (DSS)

Dextran Sulfate Sodium Salt (DSS) is a polyanionic derivative, and although the mechanism of its induction of colitis in the model is still not well understood, it is generally believed to be related to macrophage dysfunction, intestinal microbiota dysbiosis, toxic effects of DSS on the colon epithelium, and the important role of cytokines in the pathogenesis of DSS colitis model.

 

Case 1

Animal Model: rats, 1 week old, 100 g;

Procedure: 5% DSS, 3.5 mL/100 g daily gavage, gavage for 2 weeks, HE staining;

Results: The colon length of UC rats was lower than that of healthy rats. The colonic mucosa is abnormal, with a large number of inflammatory cells.

Figure 1. (a) Intestinal length of healthy rats and UC rats; (b) HE staining results of colitis sections

 

Case 2

Animal Model: rats, 8 weeks old, 200 g;

Protocol: Drink 3.5% DSS for 10 days and stain with HE;

Results: Abnormality of the colonic mucosa.

 

 

Case 3

Animal Model: rats, 7 weeks old, 300-320 g;

Protocol: After drinking DSS at 2.0% concentration for 7 days, distilled water for 7 days for a total of 4 cycles, HE staining;

Results: Abnormality of the colonic mucosa.

 

 

Product Advantages:

Yeasen Biotech provides high-quality DSS (Cat.NO: 60316ES, MW: 36000~50000), which has the following advantages:

1. The symptoms are highly similar to those of human UC: it can be used to study the mechanism and efficacy of colitis.

2. High mold forming rate: free to drink DSS aqueous solution, simple and easy to do, strong repeatability.

3. A variety of colitis models can be constructed: acute colitis, chronic colitis, and colitis-related cancer (CAC) models can also be constructed in combination with AOM.

4. Suitable for modeling a variety of animals: mice, rats, zebrafish, pigs, fruit flies, etc.

5. High safety: DSS can be degraded by natural ecosystems, which is safe for the environment;

6. High purity (>98%), sulfur content 17-19%, free sulfur <0.2%, supported by a large number of data literature, widely used in the construction of UC model;

7. Combined with the use of Azoximetana(Aum), it can be used to induce colitis-related cancer (Colidis, Asochatate Kanse, Ka) animal model, successfully simulating the process of Ibud inducing Kachi.

 

Published articles with our reagents (incomplete statistics)：

[1] Zhong D, Jin K, Wang R, Chen B, Zhang J, Ren C, Chen X, Lu J, Zhou M. Microalgae-Based Hydrogel for Inflammatory Bowel Disease and Its Associated Anxiety and Depression. Adv Mater. 2024 Jan 26: e2312275. doi: 10.1002/adma.202312275. Epub ahead of print. PMID: 38277492.  (IF: 29.4)

[2] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464. (IF: 16.74)

[3] Tong, L, et al. Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Theranostics 2021, 11 (17), 8570-8586. DOI: 10.7150/thno.62046. (IF: 11.56)  

[4] Feng X, et al. Yeast Microcapsule Mediated Natural Products Delivery for Treating Ulcerative Colitis through Anti-Inflammatory and Regulation of Macrophage Polarization. ACS Appl Mater Interfaces. 2022 Jul 13;14(27):31085-31098. doi: 10.1021/acsami.2c05642. Epub 2022 Jun 30. PMID: 35770618. (IF: 10.38)  

[5] Li X, et al. Discoidin domain receptor 1(DDR1) promote intestinal barrier disruption in Ulcerative Colitis through tight junction proteins degradation and epithelium apoptosis. Pharmacol Res. 2022 Sep;183:106368. doi: 10.1016/j.phrs.2022.106368. Epub 2022 Jul 26. PMID: 35905891. (IF: 10.33)

[6] Jingjing Gan, et al. Orally administrated nucleotide-delivery particles from microfluidics for inflammatory bowel disease treatment. Applied Materials Today, Volume 25, 2021, 101231. (IF: 10.04)

[7] Gan J, et al. Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment. Adv Healthc Mater. 2022 Sep;11(17):e2201105. doi: 10.1002/adhm.202201105. Epub 2022 Jun 30. PMID: 35737997. (IF: 9.93)

[8] Dong J, et al. Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models. Cell Rep. 2021 Oct 26;37(4):109886. doi: 10.1016/j.celrep.2021.109886. PMID: 34706245. (IF: 9.42)

[9] Li Y, et al. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes. 2020 Jul 3;11(4):789-806. doi: 10.1080/19490976.2019.1709387. Epub 2020 Jan 13. PMID: 31931652. (IF: 7.74)

[10] Xu M, et al. Identification of Immune-Related Gene Signature and Prediction of CeRNA Network in Active Ulcerative Colitis. Front Immunol. 2022;13:855645. Published 2022 Mar 22. doi:10.3389/fimmu.2022.855645. (IF:7.56)

[11] Wang S, et al. Isosteviol Sodium Exerts Anti-Colitic Effects on BALB/c Mice with Dextran Sodium Sulfate-Induced Colitis Through Metabolic Reprogramming and Immune Response Modulation. J Inflamm Res. 2021; 14:7107-7130. Published 2021 Dec 20. doi:10.2147/JIR.S344990. (IF:6.92)

[12] Liu C, et al. Angelica oil restores the intestinal barrier function by suppressing S100A8/A9 signalling in mice with ulcerative colitis. Phytomedicine. 2023; 108:154490. doi:10.1016/j.phymed.2022.154490. (IF:6.66)

[13] Wang S, et al. Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-κB Pathway. Oxid Med Cell Longev. 2022;2022:4636618. Published 2022 Jan 27. doi:10.1155/2022/4636618. (IF:6.54)

[14] Li Z, et al. A tetrapeptide from maize combined with probiotics exerted strong anti-inflammatory effects and modulated gut microbiota in DSS-induced colitis mice [published online ahead of print, 2022 Nov 14]. Food Funct. 2022;10.1039/d2fo02678c. doi:10.1039/d2fo02678c. (IF:6.32)

[15] Jia X, et al. The potential mechanism of huazhuojiedu decoction in the treatment of ulcerative colitis based on network pharmacology and experimental validation. Front Pharmacol. 2022;13:1033874. Published 2022 Oct 14. doi:10.3389/fphar.2022.1033874. (IF:5.99)

[16] Luo P, et al. Central administration of human opiorphin alleviates dextran sodium sulfate-induced colitis in mice through activation of the endogenous opioid system. Front Pharmacol. 2022;13:904926. Published 2022 Sep 13. doi:10.3389/fphar.2022.904926. (IF:5.99)

[17] Zhang K, et al. Pharmacokinetic Study of Four Major Bioactive Components of Liandan Xiaoyan Formula in Ulcerative Colitis and Control Rats Using UPLC-MS/MS. Front Pharmacol. 2022;13:936846. Published 2022 Jul 4. doi:10.3389/fphar.2022.936846. (IF:5.99)

[18] Yan G, et al. Immuno-PET Imaging of TNF-α in Colitis Using ⁸⁹Zr-DFO-infliximab. Mol Pharm. 2022;19(10):3632-3639. doi:10.1021/acs.molpharmaceut.2c00411. (IF:5.36)

[19] Xiong X, et al. HuanglianGanjiang Tang alleviates DSS-induced colitis in mice by inhibiting necroptosis through vitamin D receptor. J Ethnopharmacol. 2022;298:115655. doi:10.1016/j.jep.2022.115655. (IF:5.19)

[20] Zhu Y, et al. Endometrial Regenerative Cell-Derived Exosomes Attenuate Experimental Colitis through Downregulation of Intestine Ferroptosis. Stem Cells Int. 2022;2022:3014123. Published 2022 Aug 22. doi:10.1155/2022/3014123. (IF:5.13)

 

Product Order

Product name	Catalog No.	Size	Price(＄)
Dextran Sulfate Sodium Salt (DSS) MW:36000~50000	60316ES25	25 g	255
	60316ES60	100 g	755
	60316ES76	500 g	3455
	60316ES80	1 kg	6255