Description

This product is a Linear Polyethylenimine (PEI) after optimization and transformation, with a concentration of 1 mg/mL. This product is purely chemically synthesized and does not contain animal-derived ingredients. It is particularly suitable for co-transfection of multiple plasmids, for the production of recombinant virus vectors and the instantaneous expression of recombinant proteins. This product has low cytotoxicity, high transfection efficiency, and is compatible with antibiotics. It has high gene expression efficiency in HEK293 and other cells.

Feature

• Small scale and large-scale production of various viruse vectors and transient expression of proteins suitable for adherent and suspension systems
• High transfection efficiency, less plasmid usage and high-cost performance
• Compatible with a variety of commercial medium
• Sufficient capacity and stable batch

Application

• Cell Transfection

Specification

Physical and chemical Properties	Information
Form	Liquid
Serum Compatible	Yes
Cell Type	Established Cell Lines
Sample Type	Plasmid DNA
Transfection Technique	PEI-Based Transfection

Components

Components No.	Name	40820ES04	40820ES10	40820ES60
40820	Hieff Trans™ PEI Transfection Reagent	1.5 mL	10 mL	100 mL

Storage

The product could be stored at 2-8ºC for two years. Do not freeze!

 

The product should be stored at 2-8℃ for two years. Do not freeze!

Figures

• Quality

Item	Specifications	R&D	GMP
Clarity	Colorless liquid	√	√
Activity	Transfection efficiency>70% Cell viability>80%	√	√
Endotoxin	<0.5 EU/mL	√	√
Sterility	Sterile after 14 days	√	√
Mycoplasma	Absence	√	√
pH	<7.0		√
Osmolality	≤30 mOsm/kg		√
Impurity distribution	No residual acetonitrile, methanol, ether, toluene		√

 

• Transfection Efficiency

Figure 1. The transfection efficiency of Hieff Trans™ PEI Transfection Reagent-GMP.

Hieff Trans™ PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP expression was analyzed 48 hours post-transfection. (the ratio of the plasmid to reagent is 1:1)

• Customer's test data (AAV2)

Suspended cell 293F
Sample	Transfection efficiency	Cell viability	Cell count
YEASEN	100%	92.00%	6.80E+6 /ml
P*	100%	71.00%	6.05E+6 /ml

             Culture vessel: 30 mL medium (125 mL shake flask)

             Medium: OPM-293CD-05 serum-free medium

             Incubation time: 72 h

             Plasmid: 60 μg(MCS-GFP : RC2 : pHelper = 1 : 1 : 1 = 20 μg : 20 μg : 20 μg)

             Transfection reagent: 120 -180 μL

 

• Customer's test data (LV)

            Cell viability: 98%

            Culture vessel: 50 mL medium (250 mL shake flask)

            Cell count: 5E+6/mL

            Plasmid: 2 mg/L 

            Transfection reagent: 4-8 ml/L

            Incubation time: 48 h