10×Digestion buffer 3 GMP-grade

Details:

Description

This product is an optimized 10×Digestion buffer for different restriction enzymes to ensure optimal (100%) digestion activity. The formulation of the product: 500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl2, 1 mg/mL OsrHSA, pH 7.9 at 25°C.  This product is produced in accordance with GMP process requirements and provided in a liquid form.

Application

  • Buffer for different restriction enzymes to ensure optimal (100%) digestion activity

Specification

Format Liquid
Concentration 10X
Formulation 500 mM Tris-HCl, 1 M NaCl, 100 mM MgCl2, 1 mg/mL OsrHSA, pH 7.9 at 25°C

Components

Components No. Name 10667ES03 10667ES10 10667ES50
10667 10×Digestion buffer 3 GMP-grade 1 mL 10 mL 50 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.

Figures

Figure1. The performance of YEASEN BspQI is superior.

In a 50 μL reaction system, 1 μg of λDNA was treated with the corresponding amount of BspQI (incubate at 50 °C for 60 min and then incubate at 80 °C for 20 min to inactivate BspQI). Then 20 μL of the reaction solution was loaded.

M:  DNA marker
C: The control group without BspQI treatment

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