Description

Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit is a mRNA transcriptome library construction kit compatible with Illumina and MGI high-throughput sequencing platforms. Compared with the traditional library construction method, this product combines cDNA second strand synthesis with Endprep and dA-tailing, which greatly reduces the time for library construction and simplifies the operation. The two-strand synthesis module is equipped with two buffers to meet the need for a conventional library or strand-specific library. This product is compatible with 10 ng-4 μg of input total RNA from eukaryotes. After mRNA isolation, fragmentation, double-stranded cDNA synthesis, end repair, dA-tailing, adapter ligation, and library amplification, the total RNA sample is finally converted into a library suitable for sequencing on the Illumina or MGI platform. 
Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit contains two independent modules. The core components of BOX-I are oligo (dT) magnetic beads required for mRNA purification. BOX-II contains mRNA fragmentation reagents, reverse transcription reagents, conventional and chain-specific ds-cDNA synthesis reagents, and others required for subsequent library construction. dTTP is replaced with dUTP in the strand-specific two-strand synthesis Buffer so that dUTP is incorporated into the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, which achieves strand specificity. All reagents provided have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.

Features

• One kit, four types of libraries: Illumina platform strand specific library; Illumina platform regular RNA library; MGI platform strand specific library; Illumina platform regular RNA library;
• Streamline the process: end repair and dA-tailing are combined in one step, simplifying the operation steps
• Wide scope of application: applicable to samples containing polyA tails of RNA from different sources, total RNA 10 ng-4 μ g
• High gene detection rate
• Stable quality: strict batch performance and stability quality control

Applications

• Gene expression detection: detect the mRNA expression in the sample
• Detection of SNP, Indel, etc
• Identification of fusion gene
• Analysis of alternative splicing events

Specifications

Library type	mRNA library
mRNA isolation method	Oligo dT Beads
Strand？	Optional
Total RNA input amount	10 ng-4 μg
Library preparation total time (hr)	~5h
Instrument compatibility	Illumina or MGI Platforms
Recommended application	Gene expression

Components

Component No.		Name	12309ES24	12309ES96
BOX-I	12603-A	mRNA Capture Beads	1.2 mL	4.8 mL
	12603-B	Beads Binding Buffer	1.2 mL	4.8 mL
	12603-C	Beads Wash Buffer	15 mL	60 mL
	12603-D	Tris Buffer	1.2 mL	4.8 mL
BOX-II	12309-A	Frag/Prime Buffer	450 μL	2×900 μL
	12309-B	1st Strand Enzyme Mix	48 μL	192 μL
	12309-C	Strand Specificity Reagent	150 μL	580 μL
	12309-D	2nd Strand Buffer (dNTP)	720 μL	2×1440 μL
	12309-E	2nd Strand Buffer (dUTP)	720 μL	2×1440 μL
	12309-F	2nd Strand Enzyme Master Mix	120 μL	480 μL
	12309-G	Ligation Library length distribution detection	720 μL	2×1440 μL
	12309-H	Novel T4 DNA Ligase	120 μL	480 μL
	12309-I	2×Super Canace™ II High-Fidelity Mix	600 μL	2×1200 μL

Shipping and Storage

The Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit components in Box I are shipped with ice packs and can be stored at 2℃~8℃ for one year.
The Hieff NGS™ Ultima Dual-mode mRNA Library Prep Kit components in Box II are shipped with dry ice and can be stored at -15℃~ -25℃ for one year.

Figures

• Yeasen kit has a high and stable library yield

Figure 1 Stable library yield

• Yeasen kit has a high mapping rate, a high proportion of exons, low Dup rate, a high number of genes detected, and low auto junction rate.

Figure 2 Excellent sequencing quality