Product Description

DNA Polymerase I, in the presence of a template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity. This enzyme can be used with DNase I (Cat#10607) for nick translation reaction and with RNase H (Cat#12906) to synthesize the second strand of cDNA.

Product Components

Components		14452ES03	14452ES10	14452ES50
14452-A	DNA Polymerase I (20 U/μL)	1 mL	10 mL	50 mL

Unit Definitions

1 unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C.  

Shipping and Storage

The product is shipped with dry ice and can be stored at -20°C for two years.

Cautions

1. For your safety and health, please wear lab coats and disposable gloves for the operation.

2. This product is for research use ONLY!

Applications

Labeling 3'-termini of nucleic acids

Nick translation of DNA

Second strand synthesis of cDNA