Description

RNase inhibitor can bind RNase to form a complex, thereby specifically inactivating RNase. This product is a recombinant mouse-derived RNase inhibitor expressed and purified in E. coli in a soluble form, which can inhibit various types of RNase (RNase A, B, C). Also tested by RT-PCR and RT-qPCR. Compared with human-derived RNase inhibitor, this product does not contain the two cysteines that are very sensitive to oxidation in human-derived proteins, so it has higher antioxidant activity; and it’s more suitable for high-DTT sensitivity experiments ( such as qPCR, etc.). This product is produced in accordance with GMP process requirements and is provided in liquid form.

Feature

• Improved resistance to oxidation, compared to human/porcine RNase inhibitor
• Ideal for reactions where low DTT concentrations are required (e.g., Real-time PCR)
• Isolated from a recombinant source
• Tested for the absence of DNases and RNases

Application

• RT-PCR
• cDNA synthesis
• In vitro transcription/translation
• Enzymatic RNA labeling reaction
• Other applications where the integrity of RNA is important

Specification

Source	Recombinant E.coli with Murine RNase inhibitor gene
Storage Buffer	20 mM Hepes KOH，150 mM KCl，8 mM DTT，0.5%（v/v）NP 40，0.5%（v/v）Tween20，50%（v/v）Glycerol，pH 7.5 ±0.2 (25℃)
Unit Definition	The amount of enzyme required to inhibit 50% of the activity of 5 ng RNase A is defined as 1 activity unit

Components

Components No.	Name	10621ES10 (10 KU)	10621ES20 (20 KU)	10621ES60 (100 KU)	10621ES99 (1 MU)
10621	Murine RNase inhibitor GMP-grade (40 U/μL)	250 μL	500 μL	2.5 mL	25 mL

Shipping and Storage

The Murine RNase inhibitor GMP-grade products are shipped with dry ice and can be stored at -15℃ ~ -25℃ for one year.