Product Description

 Hieff NGS™ FFPE DNA Repair Reagent is a repair reagent designed for FFPE samples. This reagent repairs different kinds of damage in low-quality FFPE samples and improves the library yield and sequencing quality. This reagent is suitable for Yeasen Hieff NGS™ Ultima DNA Library Prep Kit for Illumina® (Cat#12199), which can repair 5 ng - 1 μg fragmented FFPE DNA without extra operation steps. It is recommended to process repairment and purification before library construction when using other brand reagents for library preparation.

Product Components

Components		12606ES24	12606ES96
12606-A	FFPE DNA Repair Mix	120 μL	480 μL
12606-B	FFPE DNA Repair Buffer	144 μL	576 μL

Shipping and Storage

All the components are shipped with dry ice and can be stored at -20°C for one year.

Cautions

1)For your safety and health, please wear lab coats and disposable gloves to operate.

2)Thaw all components of the kit at room temperature before use. After thawing, mix thoroughly upside down several times, centrifuge briefly and put on ice for later use.

3)It is recommended to pipette or gently vortex the mixture before preparing the reaction liquid at each step. Vigorous vortexing may impact the library yield.

4)It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tips when processing different samples.

5)It is highly recommended to pre-heat the lid of the thermocycler for each reaction step.

6)Improper operations may very likely cause carry-over contaminations through aerosols, impacting the experiment accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.

7)For research use only！