Description

Hieff NGS™ OnePot II DNA Library Prep Kit for MGI is a new generation of library prepare kit specially designed for MGI sequencing platform, which contains high-quality enzyme for DNA fragmentation. This kit combines fragmentation, end-repair, and dA-tailing into one step, significantly reducing the time and cost of library preparation. The kit has a high library conversion rate and can be applied to most DNA samples from animals, plants, and microorganisms, including low-quality samples such as FFPE samples.

Features

• Compatible with 10 ng-1 μg DNA samples, including gDNA and cDNA 
• High-quality fragmentation enzyme which randomly fragments dsDNA without bias
• Combining fragmentation, end-repair, and A-tailing in one step
• High fidelity polymerase with high amplification efficiency, significantly increasing library quality and yield
• Compatible with FFPE DNA samples
• Stringent quality control and batch effect elimination

Applications

• Construction of DNA library for MGI platform
• Suitable for sequencing in tumor, reproductive genetics, genetic disease detection, and other fields

Specifications

Product Type	Library Preparation Kit
Libraries	Fragment Library
Fragmentation method	Smearase
Input amount	10 ng-1 μg
For Use With (Equipment)	MGI Platforms
Sample Type	gDNA, cDNA and FFPE samples
Sequencing Type	Genome & DNA Sequencing
Product Line	DNA library constrction
For Use With (Application)	NGS library preparation
Quantity	16/96 Reactions

Components

Components No.	Name	13321ES16	13321ES96
13321-A	Smearase™ Mix	160 μL	960 μL
13321-B	Ligation Enhancer	480 μL	4×720 μL
13321-C	Fast T4 DNA Ligase	80 μL	480 μL
13321-D	2×Ultima HF Amplification Mix	400 μL	4×600 μL
13321-E	Primer Mix for MGI	80 μL	480 μL

Storage

-25℃ ~ -15℃ storage, valid for one year.