Description

The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes.
This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.

Feature

• Validated, product-specific process and analytical methods
• Product-specific stability
• Documentation follows applicable GMP guidelines
• AOF production process and raw materials (TSE & BSE) 
• Nitrosamine statement
• Regulatory support documents available
• Large-scale production
• Nucleotide in the multiple salt form（Na⁺, Tris etc) always available to meet different downstream application needs

Application

• RNA synthesis and amplification
• Building block for in vitro transcription

Component

Components No.	Name	10133ES03
10133-A	ATP Solution (100 mM）	1 mL
10133-B	UTP Solution (100 mM）	1 mL
10133-C	CTP Solution (100 mM）	1 mL
10133-D	GTP Solution (100 mM）	1 mL

Shipping and Storage

The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for two years.

Figures

• Standard RNA Synthesis

Figure 1. Standard RNA was synthesized in vitro using T7 RNA synthesis kit.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was analyzed by NanoDrop spectrophotometer as shown in Figure 1.

• Capped RNA Synthesis

Figure 2. Synthesis of capped RNA in vitro.

The reaction was incubated in PCR instrument at 37℃ for 2h, and then purified by magnetic beads (Cat#12602). The yield result was assayed by NanoDrop spectrophotometer as shown in Figure 2A. The integrity result was analyzed by capillary electrophoresis as shown in Figure 2B.