Hieff UNICON™ Hotstart J-Taq DNA polymerase is a thermostable Taq DNA Polymerase mutant complexed with a proprietary antibody that inhibits polymerase activity below 60℃. The polymerase activity can only be released after heating at 95℃, thus avoiding nonspecific amplification and primer-dimer formation during sample preparation and heating process prior to PCR cycling. Hieff UNICON™ Hotstart J-Taq DNA polymerase, like wild-type Taq DNA polymerase, also has 5′-3′ polymerase activity and 5′-3′ exonuclease activity but lacks any detectable 3′-5′ exonuclease activity.
Component number |
Component |
Cat#/Size |
||||
10723ES72(250 U) |
10723ES76(500 U) |
10723ES80(1000 U) |
10723ES92(10000 U) |
10723ES93(25000 U) |
||
10723 |
Hotstart J-Taq (5 U/μL) |
50 μL |
100 μL |
200 μL |
1 mL×2 |
1 mL×5 |
The product is shipped with ice packs and can be stored at -20°C for 2 years.
Components |
Vloume(μL) |
Final concentration |
2×Buffer |
10 |
1× |
Primer mix (10 μmol/L) |
0.2-1 |
0.1 μmol/L-0.5 μmol/L |
Probe mix (10 μmol/L) |
0.2-1 |
0.1 μmol/L-0.5 μmol/L |
Hotstart J-Taq (5 U/μL) |
0.5 |
2.5 U |
DNA Template |
100 pg-100 ng |
- |
Sterile ultra-pure water |
up to 20 |
- |
[Note]: The optimal concentration for above components can be adjusted according to the specific experimental conditions.
Cycle step |
Temperature |
Time |
Cycles |
Initial denaturation |
95℃ |
5 min |
1 |
denaturation annealing/extension |
95℃ 60℃ |
15 sec 30 sec |
40 |
[Note]: The annealing/extension temperature can be appropriately adjusted according to the experimental requirements.
For your safety and health, please wear lab coats and disposable gloves for operation.
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