Hieff Unicon™ Universal Blue qPCR Master Mix (SYBR Green Based)



Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) is a pre-solution for 2×real-time quantitative PCR amplification characterized by high sensitivity and specificity, is blue in color, and has the effect of sample addition tracing. The core component Hieff Unicon™ Taq DNA polymerase uses antibody hot start to effectively inhibit non-specific amplification due to primer annealing during sample preparation. At the same time, the formula adds the promoting factors to improve the amplification efficiency of PCR reaction and equalize the amplification of genes with different GC contents (30 ~ 70%), so that quantitative PCR can obtain a good linear relationship in a wide quantitative region. This product contains special ROX Passive Reference Dye, which is applicable to most qPCR instruments. It is not necessary to adjust the concentration of ROX on different instruments. It is only necessary to add primers and templates to prepare the reaction system for amplification.


  • Built-in visual indicator: It contains an inert blue dye that does not interfere with real-time PCR and provides higher visibility in tubes or Wells.
  • High specificity: It contains Taq DNA polymerase and is tightly controlled by an antibody-mediated heat-priming mechanism, thereby avoiding nonspecific amplification.
  • Wide instrument compatibility: It is compatible with most qPCR instruments and eliminates the need to adjust the concentration of ROX on different instruments


  • Gene Expression


Detection method Fluorescence
PCR method qPCR
Polymerase Taq DNA polymerase
Type of sample DNA
Application equipment Most qPCR instruments
Product Type SYBR premix for real-time fluorescence quantitative PCR
Apply to (application) Gene Expression


Components No. Name 11184ES03 11184ES08 11184ES50 11184ES60
11184 Hieff Unicon™ Universal Blue qPCR Master Mix (Dye Based) 1 mL 5×1 mL 50×1 mL 100×1 mL

Shipping and Storage

The product is shipped with ice packs and can be stored at -15℃ ~ -25℃ for 18 months. The product contains fluorescent dyes, so it is necessary to avoid strong light irradiation when storing or preparing the reaction system.


Figure 1. High sensitivity: the ability to detect a single copy.


Figure2. High resolution & Excellent reproducibility of duplicate wells.


 Figure3. Excellent detection rate and specificity: broadly suited for the amplification of 30-70% GC.

Cited from "SARS-CoV-2 envelope protein causes acute respiratory distress syndrome (ARDS)-like pathological damages and constitutes an antiviral target. Cell Res. 2021;31(8):847-860. doi:10.1038/s41422-021-00519-4"

Cited from "A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens. Cell . 2020 Sep 3;182(5):1109-1124.e25. doi: 10.1016/j.cell.2020.07.020. "

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