Hieff™ Taq DNA Polymerase

Details:

Description

Hieff™ Taq DNA polymerase is a thermostable recombinant DNA polymerase expressed by Thermus aquaticus with a molecular weight of 94 KDa. It has 5'→3' polymerase activity and 5'→3' exonuclease activity but without 3'→5' exonuclease activity. The amplified product has 3'- dA and can be directly used for TA cloning.

Features

  • 5'→3' DNA polymerase activity
  • 5'→3' exonuclease activity
  • Hieff™ Taq DNA polymerase is an ideal tool for standard PCR with templates of 5 kb or less

Applications

Genotyping, colony PCR and other conventional PCR

Specifications

Polymerase Taq DNA polymerase
Molecular Weight 94 KDa
Unit Definition Using the activated DNA of salmon sperm as template/primer, the activity is defined as one active unit (U) when 10 nmol of total nucleotide was ingested as acid insoluble substance at 74℃ for 30 min.

Components

Components No. Name 10101ES80 (1, 000 U) 10101ES92 (10, 000 U)
10101-A 10×Taq Buffer (Mg2+ Free) 4×1 mL 4×10 mL
10101-B 25 mM MgCl2 2×1 mL 2×10 mL
10101-C Hieff™ Taq DNA Polymerase (5 U/μL) 200 μL 2×1 mL

Storage

The product should be stored at -25℃ ~ 15℃ for two years.

Documents:
Citations & References:

[1] Lu Z, Yang S, Yuan X, et al. CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis. Nucleic Acids Res. 2019;47(7):e40. doi:10.1093/nar/gkz072(IF:11.147)

[2] Chen X, Liang H, Xi Z, et al. BM-MSC Transplantation Alleviates Intracerebral Hemorrhage-Induced Brain Injury, Promotes Astrocytes Vimentin Expression, and Enhances Astrocytes Antioxidation via the Cx43/Nrf2/HO-1 Axis. Front Cell Dev Biol. 2020;8:302. Published 2020 May 8. doi:10.3389/fcell.2020.00302(IF:5.186)

[3] Fu C, Xu J, Jia Z, Yao K, Chen X. Cataract-causing mutations L45P and Y46D promote γC-crystallin aggregation by disturbing hydrogen bonds network in the second Greek key motif. Int J Biol Macromol. 2021;167:470-478. doi:10.1016/j.ijbiomac.2020.11.158(IF:5.162)

[4] Chen X, Xu CX, Liang H, et al. Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway. Aging (Albany NY). 2020;12(7):6306-6323. doi:10.18632/aging.103025(IF:4.831)

[5] Fu C, Xu J, Yang X, Chen X, Yao K. Cataract-causing mutations L45P and Y46D impair the thermal stability of γC-crystallin. Biochem Biophys Res Commun. 2021;539:70-76. doi:10.1016/j.bbrc.2020.12.096(IF:3.575)

[6] Fei ZY, Wang WS, Li SF, et al. High expression of the TEFM gene predicts poor prognosis in hepatocellular carcinoma. J Gastrointest Oncol. 2020;11(6):1291-1304. doi:10.21037/jgo-20-120(IF:2.536)

[7] Zhang L, Li Y, Bao H, et al. Population structure and antimicrobial profile of Staphylococcus aureus strains associated with bovine mastitis in China. Microb Pathog. 2016;97:103-109. doi:10.1016/j.micpath.2016.06.005(IF:1.888)

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